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anti gap43  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti gap43
    Anti Gap43, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gap43/pmc12992974-282-59-61
    Average 86 stars, based on 1 article reviews
    anti gap43 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    other:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: The following antibodies were used: anti-iNOS ( Ab178945 , abcam, USA), CD206 (E6TSJ, Cell Signaling Technology, USA), anti-IL-6 (bs-0782R, Bioss, Beijing, China), anti-TNF-α (YT4689, ImmunoWay, USA), anti-IL-1β (AF02479, AiFang biological, China), anti-GFAP (MA009369AOm, cusabio, China), anti-Iba-1 (17198, Cell Signaling Technology, USA), anti-HMGB1 (3935, Cell Signaling Technology, USA), anti-NeuN (24307, Cell Signaling Technology, USA), anti-β3-tubulin (45058, Cell Signaling Technology, USA), anti-GAP43 (8945, Cell Signaling Technology, USA), anti-CD31 (77699, Cell Signaling Technology, USA), Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (715-155-150, Jackson Immunoresearch, USA), CyTM3 AffiniPure Donkey Anti-Rabbit IgG (711-165-152, Jackson Immunoresearch, USA).

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: The following antibodies were used: anti-iNOS (Ab178945, abcam, USA), CD206 (E6TSJ, Cell Signaling Technology, USA), anti-IL-6 (bs-0782R, Bioss, Beijing, China), anti-TNF-α (YT4689, ImmunoWay, USA), anti-IL-1β (AF02479, AiFang biological, China), anti-GFAP (MA009369AOm, cusabio, China), anti-Iba-1 (17198, Cell Signaling Technology, USA), anti-HMGB1 (3935, Cell Signaling Technology, USA), anti-NeuN (24307, Cell Signaling Technology, USA), antiβ3-tubulin (45058, Cell Signaling Technology, USA), anti-GAP43 (8945, Cell Signaling Technology, USA), anti-CD31 (77699, Cell Signaling Technology, USA), Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (715-155-150, Jackson Immunoresearch, USA), CyTM3 AffiniPure Donkey Anti-Rabbit IgG (711-165-152, Jackson Immunoresearch, USA).

    Article Title: The role of astrocyte-derived extracellular vesicles in cellular microenvironment remodeling after spinal cord injury: A study based on quantitative proteomics analysis.
    Article Snippet: After spinal cord injury (SCI), astrocytes (AS), the most abundant glial cells in the central nervous system, closely interact with other nerve cells.. The precise mechanism by which astrocytes remodel the cellular microenvironment (CME) remains unclear; however, the extracellular vesicles (EVs) they release may facilitate communication between cells by transporting biological macromolecules.. This study aimed to elucidate the role of astrocyte-derived EVs in modulating CME after SCI.

    Article Title: Columbianadin Improves M1 Polarization of Microglia After Spinal Cord Injury by Stabilizing PTEN and Inhibiting the PI3K/AKT Pathway
    Article Snippet: Background: Inhibiting the polarization of microglia toward the M1 pro-inflammatory phenotype to alleviate neuroinflammation and improve neuronal survival is a critical strategy for promoting spinal cord injury (SCI) repair.. PTEN, a core regulator of cellular inflammatory responses, exerts its function by negatively modulating the PI3K/AKT signaling pathway.. Columbianadin (CBN), a coumarin derivative isolated from the roots of Angelica sinensis (Oliv.)

    Incubation:

    Article Title: Indole-3-propionic acid promotes Schwann cell proliferation following peripheral nerve injury by activating the PI3K/AKT pathway.
    Article Snippet: The sections were then blocked with a blocking solution (Beyotime Institute of Biotechnology, China, P0260) for 30min. .. Next, the tissue sections were incubated overnight at 4 C with primary antibodies, including anti-SOX10 (1:200, Proteintech, mouse), anti-Ki67 (1:200, Abmart, rabbit) and anti-GAP43 (1:200, Cell Signalling, rabbit) antibodies. .. After three washes with PBST, the sections were incubated with either a DyLight 488-labelled goat anti-rabbit (ab150077, Abcam, 1:200) or DyLight 594-labelled goat antimouse fluorescent secondary antibody (E032410-01, EarthOx, 1:200) for 2 h in the dark at room temperature.

    Article Title: Histone H3K9 lactylation activates the TXNIP/NLRP3 pathway to drive macrophage inflammation after spinal cord injury
    Article Snippet: .. Samples were blocked in 5% BSA in PBS for 30 min at room temperature, then incubated overnight at 4 °C with primary antibodies diluted in PBS: anti- F4/80 (ab6640, Abcam, 1:400), anti-IBA1 (011-27991, WAKO, 1:400), anti- Pankla (PTM-1401, PTM Bio, 1:50), anti- NLRP3 (MA5-32255, Invitrogen, 1:200), anti- TXNIP (MA5-32771, Invitrogen, 1:100), anti-β3-Tubulin (D71G9, CST, 1:400), anti-Neurofilament-L (C28E10, CST, 1:400), anti- GAP43 (D9C8, CST, 1:400) and anti- H3K9la (PTM-1421RM, PTM Bio, 1:50). ..

    Article Title: Indole-3-propionic acid promotes Schwann cell proliferation following peripheral nerve injury by activating the PI3K/AKT pathway
    Article Snippet: The sections were then blocked with a blocking solution (Beyotime Institute of Biotechnology, China, P0260) for 30 min. .. Next, the tissue sections were incubated overnight at 4 °C with primary antibodies, including anti-SOX10 (1:200, Proteintech, mouse), anti-Ki67 (1:200, Abmart, rabbit) and anti-GAP43 (1:200, Cell Signalling, rabbit) antibodies. .. After three washes with PBST, the sections were incubated with either a DyLight 488-labelled goat anti-rabbit (ab150077, Abcam, 1:200) or DyLight 594-labelled goat anti-mouse fluorescent secondary antibody (E032410-01, EarthOx, 1:200) for 2 h in the dark at room temperature.

    Article Title: Histone H3K9 lactylation activates the TXNIP/NLRP3 pathway to drive macrophage inflammation after spinal cord injury.
    Article Snippet: .. Samples were blocked in 5% BSA in PBS for 30 min at room temperature, then incubated overnight at 4 ◦C with primary antibodies diluted in PBS: anti- F4/80 (ab6640, Abcam, 1:400), anti-IBA1 (011-27991, WAKO, 1:400), anti- Pankla (PTM-1401, PTM Bio, 1:50), anti- NLRP3 (MA5-32255, Invitrogen, 1:200), antiTXNIP (MA5-32771, Invitrogen, 1:100), anti-β3-Tubulin (D71G9, CST, 1:400), anti-Neurofilament-L (C28E10, CST, 1:400), anti- GAP43 (D9C8, CST, 1:400) and anti- H3K9la (PTM-1421RM, PTM Bio, 1:50). ..



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    Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), <t>Gap43</t> immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
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    Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), <t>Gap43</t> immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
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    Proteintech protein 43
    Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), <t>Gap43</t> immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
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    Proteintech antibodies gap43
    Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), <t>Gap43</t> immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
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    Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), <t>Gap43</t> immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
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    Image Search Results


    Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), Gap43 immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

    Journal: Materials Today Bio

    Article Title: Injectable anti-inflammatory, antioxidant supramolecular nanofiber hydrogel for peripheral nerve injury repair and neuropathic pain relief

    doi: 10.1016/j.mtbio.2026.102780

    Figure Lengend Snippet: Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), Gap43 immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

    Article Snippet: The antibodies used and their dilution ratio include: NF-κB (CST, 1:1000), P- NF-κB (CST, 1:1000), Gap43 (CST, 1:1000), S100β (Abcam, 1:1000), GAPDH (Proteintech, 1:1000).

    Techniques: Staining, Immunohistochemistry, Western Blot, Expressing, Software